Application

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Immunoprecipitation (Use Protein A): Ab 10µg/mg protein lysate.

Western Blotting: Ab 5µg/ml for 2hrs at room temperature.

Optimal working dilutions must be determined by end user.

This Anti-Amphiregulin Antibody is validated for use in ELISA, IP, WB for the detection of Amphiregulin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A synthetic 19-mer peptide, corresponding to aa 26-44 from the secreted amphiregulin of human origin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin from rabbit anti-serum by Protein A chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.

Format: Purified

Specificity

Recognizes a glycoprotein of 18.5-22.5kDa, identified as amphiregulin (AR). Antibody is directed against the region of AR, which binds to heparin and is highly specific to AR. It shows no cross-reaction with EGF, TGFalpha, or HB-EGF. In vitro, AR functions as an autocrine growth stimulator to derive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. AR is important in development of human placenta and murine mammary gland.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.